| Code: | F B01 |
| Department: | Department of Biophysics |
| Course director: | Péter Maróti |
| Credits: | 2 |
| Semester: | not fixed |
| Hours/week: | 2+0 |
| Prerequisities: | Biophysics, Spectroscopy 1. |
| Type of assessment: | K or B |
Course description:
Basic principles and methods of absorbance
and fluorescence. Techniques and applications. Light spectroscopy of amino
acids, nucleic acids, proteins, and chromophores. Photosynthesis. Light-converting
proteins. Biophotons: correlation with biological and physiological function.
Probing the environment: environmentally sensitive chromophores, ligand
binding to proteins, typical optical probes, measurements of ion concentration
inside the cell, intravesicular pH and transmembrane potential. Fluorescence
energy transfer as a molecular ruler. Probing the structure of a four-way
DNA junction.
Recommended Texts:
Table of Courses |