| Article |
| Influence of arbuscular mycorrhiza and cadmium on the polyamine contents of Ri T-DNA transformed Daucus carota L. root cultures |
| István Parádi*, Bernadett Berecz, Krisztián Halász, Zoltán Bratek |
| Department of Plant Physiology, Eötvös Loránd University, Budapest, Hungary |
| Influence of arbuscular mycorrhizal (AM) colonisation on free polyamine (PA) contents and ratios was investigated in vitro by Ri T-DNA transformed root cultures (Daucus carota L. and Glomus intraradices Schenck & Smith) under Cd-treatment. Roots were soaked in Cd(NO3)2 solutions of 3x10-6 M and 5x10-5 M concentrations for 6 hours. Roots were strongly colonised and their Cd-content increased in case of the higher Cd-treatment only. In contrast to earlier results, Cd treatment reduced putrescine and spermidine contents of the non-mycorrhizal (NM) Daucus carota roots, whereas that of mycorrhizal (M) roots did not change. Spermine content showed a slight increase in all cases. NM roots always had higher PA ratios. AM symbiosis may have established a more equalised environment for the roots and so decreased the volume of physiological responses induced by stress conditions, which are normally reflected by strong changes in polyamine contents. Acta Biol Szeged 47(1-4):31-36 (2003) PDF |
| Key Words: arbuscular mycorrhiza, cd stress, polyamine, putrescine, root culture, spermidine |
| *Corresponding author. E-mail: iparadi@hotmail.com |